supernatant

Tubes were put on the magnet until the supernatant ran clear so that it could be removed and discarded.

The detection limit in the plaque assay is 10 p.f.u. ml−1 in cell supernatant, and 100 p.f.u. ml−1 in serum.

After lysis, the lysate was centrifuged at 13,200 r.p.m. for 15 min, the supernatant was transferred to fresh tubes, and a second round of centrifugation was performed.

Equal volumes of the input, pellet and post-centrifugation supernatant fractions were then analysed by western blot, as described above.

Cells were cultured in HMM for 24 h, and the supernatant was collected for analysis.